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The Resource Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)

Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)

Label
Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach
Title
Biochemistry Laboratory Manual For Undergraduates
Title remainder
An Inquiry-Based Approach
Statement of responsibility
Timea Gerczei Fernandez, Scott Pattison
Creator
Contributor
Author
Subject
Language
  • eng
  • eng
Summary
Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.--
Assigning source
Provided by publisher
Cataloging source
DE-B1597
http://library.link/vocab/creatorName
Gerczei Fernandez, Timea,
Government publication
other
Language note
In English
Nature of contents
dictionaries
http://library.link/vocab/relatedWorkOrContributorName
Pattison, Scott
Series statement
Open Access e-Books
http://library.link/vocab/subjectName
  • Biochemie
  • Labortechnik
Target audience
specialized
Label
Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
Link
https://doi.org/10.2478/9783110411331
Instantiates
Publication
Copyright
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
  • 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
  • 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
  • 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
  • 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
  • 11 Understanding the Importance of Buffers in Biological Systems
  • 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
  • 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
  • 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
  • List of Figures
  • Frontmatter
  • Preface
  • Contents
  • 1 Introducing the Bacterial Antibiotic Sensor Mini Project
  • 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
  • 3 Designing Primers for Site-Directed Mutagenesis
  • 4 Performing Site-Directed Mutagenesis
  • 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
Control code
9783110411331
Extent
1 online resource
Form of item
online
Isbn
9783110411331
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.2478/9783110411331
Specific material designation
remote
System control number
(OCoLC)1110450072
Label
Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
Link
https://doi.org/10.2478/9783110411331
Publication
Copyright
Carrier category
online resource
Carrier category code
  • cr
Carrier MARC source
rdacarrier
Content category
text
Content type code
  • txt
Content type MARC source
rdacontent
Contents
  • 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
  • 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
  • 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
  • 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
  • 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
  • 11 Understanding the Importance of Buffers in Biological Systems
  • 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
  • 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
  • 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
  • List of Figures
  • Frontmatter
  • Preface
  • Contents
  • 1 Introducing the Bacterial Antibiotic Sensor Mini Project
  • 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
  • 3 Designing Primers for Site-Directed Mutagenesis
  • 4 Performing Site-Directed Mutagenesis
  • 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
Control code
9783110411331
Extent
1 online resource
Form of item
online
Isbn
9783110411331
Media category
computer
Media MARC source
rdamedia
Media type code
  • c
Other control number
10.2478/9783110411331
Specific material designation
remote
System control number
(OCoLC)1110450072

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