The Resource Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
Resource Information
The item Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in European University Institute.This item is available to borrow from 1 library branch.
Resource Information
The item Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource) represents a specific, individual, material embodiment of a distinct intellectual or artistic creation found in European University Institute.
This item is available to borrow from 1 library branch.
- Summary
- Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.--
- Language
-
- eng
- eng
- Extent
- 1 online resource
- Contents
-
- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- 11 Understanding the Importance of Buffers in Biological Systems
- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures
- Frontmatter
- Preface
- Contents
- 1 Introducing the Bacterial Antibiotic Sensor Mini Project
- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
- 3 Designing Primers for Site-Directed Mutagenesis
- 4 Performing Site-Directed Mutagenesis
- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
- Isbn
- 9783110411331
- Label
- Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach
- Title
- Biochemistry Laboratory Manual For Undergraduates
- Title remainder
- An Inquiry-Based Approach
- Statement of responsibility
- Timea Gerczei Fernandez, Scott Pattison
- Language
-
- eng
- eng
- Summary
- Biochemistry laboratory manual for undergraduates - an inquiry based approach by Gerczei and Pattison is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology while incorporating the blossoming field of bioinformatics. The novelty of this manual is the incorporation of a student-driven real real-life research project into the undergraduate curriculum. Since students test their own mutant design, even the most experienced students remain engaged with the process, while the less experienced ones get their first taste of biochemistry research. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.--
- Assigning source
- Provided by publisher
- Cataloging source
- DE-B1597
- http://library.link/vocab/creatorName
- Gerczei Fernandez, Timea,
- Government publication
- other
- Language note
- In English
- Nature of contents
- dictionaries
- http://library.link/vocab/relatedWorkOrContributorName
- Pattison, Scott
- Series statement
- Open Access e-Books
- http://library.link/vocab/subjectName
-
- Biochemie
- Labortechnik
- Target audience
- specialized
- Label
- Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- 11 Understanding the Importance of Buffers in Biological Systems
- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures
- Frontmatter
- Preface
- Contents
- 1 Introducing the Bacterial Antibiotic Sensor Mini Project
- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
- 3 Designing Primers for Site-Directed Mutagenesis
- 4 Performing Site-Directed Mutagenesis
- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
- Control code
- 9783110411331
- Extent
- 1 online resource
- Form of item
- online
- Isbn
- 9783110411331
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.2478/9783110411331
- Specific material designation
- remote
- System control number
- (OCoLC)1110450072
- Label
- Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
- Carrier category
- online resource
- Carrier category code
-
- cr
- Carrier MARC source
- rdacarrier
- Content category
- text
- Content type code
-
- txt
- Content type MARC source
- rdacontent
- Contents
-
- 6 Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- 7 Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- 8 Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV spectrometry
- 9 Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluorescent Quenching
- 10 Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- 11 Understanding the Importance of Buffers in Biological Systems
- 12 Molecular Visualization of an Enzyme, Acetylcholinesterase
- 13 Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experiment
- 14 Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures
- Frontmatter
- Preface
- Contents
- 1 Introducing the Bacterial Antibiotic Sensor Mini Project
- 2 Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are Important for Function
- 3 Designing Primers for Site-Directed Mutagenesis
- 4 Performing Site-Directed Mutagenesis
- 5 Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel Electrophoresis and UV Spectroscopy
- Control code
- 9783110411331
- Extent
- 1 online resource
- Form of item
- online
- Isbn
- 9783110411331
- Media category
- computer
- Media MARC source
- rdamedia
- Media type code
-
- c
- Other control number
- 10.2478/9783110411331
- Specific material designation
- remote
- System control number
- (OCoLC)1110450072
Library Links
Embed (Experimental)
Settings
Select options that apply then copy and paste the RDF/HTML data fragment to include in your application
Embed this data in a secure (HTTPS) page:
Layout options:
Include data citation:
<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.eui.eu/portal/Biochemistry-Laboratory-Manual-For-Undergraduates/YZLWz2OtAQ4/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.eui.eu/portal/Biochemistry-Laboratory-Manual-For-Undergraduates/YZLWz2OtAQ4/">Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.eui.eu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.eui.eu/">European University Institute</a></span></span></span></span></div>
Note: Adjust the width and height settings defined in the RDF/HTML code fragment to best match your requirements
Preview
Cite Data - Experimental
Data Citation of the Item Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)
Copy and paste the following RDF/HTML data fragment to cite this resource
<div class="citation" vocab="http://schema.org/"><i class="fa fa-external-link-square fa-fw"></i> Data from <span resource="http://link.library.eui.eu/portal/Biochemistry-Laboratory-Manual-For-Undergraduates/YZLWz2OtAQ4/" typeof="Book http://bibfra.me/vocab/lite/Item"><span property="name http://bibfra.me/vocab/lite/label"><a href="http://link.library.eui.eu/portal/Biochemistry-Laboratory-Manual-For-Undergraduates/YZLWz2OtAQ4/">Biochemistry Laboratory Manual For Undergraduates : An Inquiry-Based Approach, Timea Gerczei Fernandez, Scott Pattison, (electronic resource)</a></span> - <span property="potentialAction" typeOf="OrganizeAction"><span property="agent" typeof="LibrarySystem http://library.link/vocab/LibrarySystem" resource="http://link.library.eui.eu/"><span property="name http://bibfra.me/vocab/lite/label"><a property="url" href="http://link.library.eui.eu/">European University Institute</a></span></span></span></span></div>
